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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Blood Banking | 15–20% | - Transfusion practice and testing - Blood group systems and immunology - Blood products and components |
| Topic 2: Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
| Topic 3: Chemistry | 20–25% | - Electrolytes, acid-base, and toxicology - Carbohydrates, lipids, and enzymes |
| Topic 4: Hematology | 20–25% | - Physiology and disease states - Laboratory testing and hemostasis |
| Topic 5: Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Topic 6: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 7: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
As a general rule, CSF glucose is about two thirds of the serum glucose measured in a normal adult.
Chemistry
Which of the following would be considered a normal cerebrospinal fluid glucose if the serum glucose is 70 mg/dl?
A. 60 mg/dl
B. 45 mg/dl
C. 100 mg/dl
D. 30 mg/dl
E. 70 mg/dl
Question 2
Creatinine clearance tests are utilized to estimate the glomerular function of the kidney. The creatitine clearance calculation is defined as the volume of plasma that is cleared of creatinine by the kidney per unit of time and uses the following formula:
creatinine clearance = Urine creatinine conc x Volume / Plamsa creatinine conc Creatinine clearance measurements have a recommended specimen requirement = 24-hour urine collection.
Which of the following tests would be useful in the assessment of glomerular filtration:
A. 24 hour urine protein
B. PSP test
C. Creatinine clearance
D. Urea clearance
Question 3
In radioimmunassay, a fixed concentration of labeled antigen is incubated with a constant amount of antiserum such that the concentration of antigen binding sites on the antibody is limiting. If unlabeled antigen is added to this system, there is competition between labeled antigen and unlabeled antigen for the limited and constant number of binding sites on the antibody, and thus the amount of labeled antigen bound to antibody will decrease as the concentration of unlabeled antigen increases.
Which of the following choices is correct when describing the principles of competitive radioimmunoassay procedures?
A. antibody will react preferentially with the labeled antigen
B. antibody will react preferentially with the unlabeled antigen
C. antibody will react with labeled antigen only
D. antibody will react equally with labeled and unlabeled antigen
E. antibody will react directly with radioactive labeled immunoglobulin
Question 4
hs-CRP is a more sensitive version of the C-reactive protein (CRP) test, a test that has been used for many years to assess inflammation in settings such as lupus, transplantation, infection, etc.
Which of the following cardiovascular risk markers is a more sensitive version of a test that is used to assess inflammation?
A. ApoB/ApoA1
B. hs-CRP
C. Oxidized-LDL
D. LpPLA2
Question 5
Provide the equivalent measurement for 1 centimeter.
Question options:
A. 1 kilometer
B. 10 millimeters
C. 1 inch
D. 5 millimeters
Solutions:
| Question 1 Answer: D | Question 2 Answer: B | Question 3 Answer: D | Question 4 Answer: B | Question 5 Answer: B |




