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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Calculation:
Cells Counted (in this case the average of both sides) X dilution factor (in this case 100) / # of squares counted (in this case 9) X area of each square (1mm2) X 0.1mm (depth factor) So, in this problem:
158 x 100 / 9 x 1 x 0.1mm = 17555.55/mm3 (can be converted to 17.5 x 109/L*)
*There are 1,000,000 mm3 in a liter (L). So 17555.55 X 1,000,000 = 17.5 x 109/L A manual white blood cell count was performed by the hematology technologist. The cell counts for both sides were 152 and 164 respectively. All nine large squares were counted on each side. The dilution for this kit was pre-measured at 1:100. What should the technologist report as the white cell count?

A) 175 x 10^9/L
B) 17.5 x 10^9/L
C) 1.75 x 10^9/L
D) 177.5 x 10^9/L

2. A negative glucose oxidase test and a positive test for reducing sugars in urine indicates the presence of a non-glucose reducing sugar such as galactose, fructose, glyceraldehyde, etc. This is the reason that certain patient populations require additional confirmatory sugar testing on their urine samples.
A negative glucose oxidase test and a positive test for reducing sugars in urine indicates:

A) The presence of a non-glucose reducing sugar such as galactose
B) True glycosuria
C) A false-negative glucose oxidation reaction

3. The Western Blot Assay is used as a confirmatory test for which of the following:

A) Anti-HIV-1
B) CMV Antibody
C) Anti-HCV
D) HBsAg

4. This scenario's answer can be calculated by first deciding what the total quality control labor costs are as well as what the total consumable costs are. In this case, if quality control is run 3 times per day, a total of 1095 quality control runs are performed each year. The direct labor cost of $2.63 multiplied by 1095 quality control runs equals $2879.85 per year in quality control direct labor. The hospital pays $354.00 per month on quality control consumables, which equals $4248.00 per year. The total quality control costs in a year are equal to
$2879.85 + $4248.00 = $7127.85. If 76,000 new chemistry tests panels are peformed each year, the total quality control cost per new chemistry test panel will be $0.09.
General Hospital is considering the addition of a new chemistry panel containing 12 tests. The laboratory is asked to calculate the total cost of quality control per new chemistry test panel. Quality control must be performed 3 times per day (every 8 hours). The labor cost per quality control test for this panel is $2.63. A month's worth of quality control reagent costs $354.00. What is the total quality control cost per new chemistry test panel if 76,000 of these new panels are performed each year?

A) $0.09
B) $0.04
C) $0.01
D) $1.70

5. An antithetical relationship exists between the antigens M and N. Antithetical relationships occur in situations where for a given locus, only one of two genes may be inherited, the alleles are termed antithetical alleles.
In immunohematology, an antithetical relationship exists between M antigen and which of these antigens?

A) N
B) K
C) Vw
D) S

Solutions: